基于氧化應(yīng)激探討松齡血脈康對自發(fā)性高血壓大鼠血管的保護(hù)機(jī)制

　　【摘要】探討松齡血脈康膠囊對高血壓狀態(tài)下大鼠氧化應(yīng)激損傷的保護(hù)作用及機(jī)制。將自發(fā)性高血壓大鼠隨機(jī)分為模型組、氯沙坦鉀組、松齡血脈康高劑量組和、松齡血脈康常規(guī)劑量組，Wistar Kyoto大鼠（WKY）作為正常對照組，連續(xù)給藥9周。生化法檢測血清中氧化應(yīng)激因子NO濃度、SOD活力、MDA含量。以Masson染色觀察藥物對大鼠主動脈病理組織形態(tài)學(xué)影響。借助qPCR陣列技術(shù)檢測氧化應(yīng)激相關(guān)因子RNA水平的變化，繼而借助String及KEGG Pathway數(shù)據(jù)庫對差異基因進(jìn)行網(wǎng)絡(luò)分析。與對照組比較，高血壓模型組血清NO濃度明顯降低（P<0.01），MDA濃度明顯升高（P<0.05），提示自發(fā)性高血壓大鼠血清氧化能力增強(qiáng)。給藥后，與模型組比較，氯沙坦鉀組、松齡血脈康大劑量組、松齡血脈康中劑量組MDA濃度均顯著降低（P均<0.001）。各組間SOD活力未見明顯變化（P>0.05）。qPCR陣列檢測結(jié)果顯示，與模型組比較，高劑量松齡血脈康組共得到9個(gè)差異倍數(shù)大于2的基因，其中Gch1、FOS、IGF1R基因表達(dá)量上調(diào)，PIK3CG、GPx2、CAV1、SOD1、PTEN、HSPA1B表達(dá)量下調(diào)。其中CAV1和IGF1R與氧化應(yīng)激密切相關(guān)，松齡血脈康膠囊可能通過調(diào)節(jié)CAV1和IGF1R基因表達(dá)，減輕高血壓狀態(tài)下大鼠主動脈氧化應(yīng)激損傷。
　　【關(guān)鍵詞】松齡血脈康膠囊；自發(fā)性高血壓大鼠；氧化應(yīng)激
　　【中圖分類號】R544.1 【文獻(xiàn)標(biāo)識碼】A 【文章編號】ISSN.2095-6681.2018.6..04
　　【Abstract】To investigate the protective effect and mechanism of Songling Xuemaikang Capsule（SXC）on oxidative stress injury in spontaneously hypertensive rats（SHR）.SHR were randomly divided into model group，high dose SXC group （H-SXC），normal dose SXC group （ N-SXC） and losartan group，and WKY rats served as control.Rats were administered continuously for 9 weeks.Oxidative stress related factors including NO，SOD and MDA were detected by biochemical method.Masson staining was used to observe pathological morphology of the aorta.QPCR array technique was used to detect changes of oxidative stress related factors， and then network of differential genes were analyzed with String and KEGG Pathway databases. Compared with the control group， NO concentration of model group was significantly descended （P<0.01），and the concentration of MDA increased obviously （P<0.05）.Compared with model group，concentration of MDA in losartan group，H-SXC and N-SXC were decreased significantly（P<0.001）.There was no statistical significance for SOD activity in each group （P>0.05）.75 variable genes were screened through qPCR array detection.Compared with the model group，H-SXC group received 9 differentially expressed genes，which fold change was more than 2.And Gch1， FOS and IGF1R gene expression were up regulated， while PIK3CG， GPx2， CAV1，SOD1，PTEN and HSPA1B were down regulated.H-SXC can alleviate oxidative stress by regulating genes expression of CAV1 and IGF1R in hypertensive rats.
　　【Key words】Songling xuemaikang capsule；Spontaneously hypertensive rats；oxidative stress
　　血管收縮和舒張功能障礙是血壓異常的關(guān)鍵環(huán)節(jié)，氧化應(yīng)激是血管損傷的重要因素。越來越多的研究提示，氧化應(yīng)激參與高血壓病的發(fā)生和發(fā)展，其機(jī)制可能與氧化損傷血管內(nèi)皮，影響血管功能有關(guān)[1-2]。這也提示，藥物通過調(diào)節(jié)血管氧化應(yīng)激水平、恢復(fù)血管功能，可能成為治療高血壓病新的探索方向。本課題組前期實(shí)驗(yàn)結(jié)果表明松齡血脈康膠囊能降低自發(fā)性高血壓大鼠的血壓[3]，同時(shí)提示其降壓作用可能與抗氧化應(yīng)激有關(guān)。故本研究借助qPCR陣列技術(shù)及String數(shù)據(jù)庫，以自發(fā)性高血壓大鼠為模型，進(jìn)一步研究松齡血脈康對血管的保護(hù)作用，以期為松齡血脈康的臨床應(yīng)用提供參考。

相關(guān)熱詞搜索：自發(fā)性 應(yīng)激 高血壓 血脈 氧化