煙草茄尼醇含量的遺傳分析

　　摘要：為探索與煙葉中茄尼醇含量相關(guān)基因的遺傳模式，以低茄尼醇含量煙草種質(zhì)Maryland609為母本、高茄尼醇含量煙草種質(zhì)K326為父本配制雜交組合，建立P1、P2、F1、F2的4個世代遺傳分析群體，利用UPLC（超高效液相色譜）進(jìn)行茄尼醇的定量檢測，通過“主基因+多基因”混合遺傳模型多世代聯(lián)合分析對與茄尼醇含量相關(guān)的基因進(jìn)行遺傳分析。結(jié)果表明，Maryland609×K326組合的茄尼醇含量受2對等顯性主基因+加性顯性多基因（E6）控制，2對主基因的加性效應(yīng)和顯性效應(yīng)值相等，均為負(fù)值，多基因的顯性效應(yīng)大于加性效應(yīng)；主基因的遺傳率分別為33.61%和53.15%，多基因遺傳率分別為30.01%和13.64%，環(huán)境變異在33.21%～36.28%。因此，主基因和多基因共同決定了煙葉茄尼醇的含量，以主基因遺傳為主，同時受到環(huán)境的影響。
　　關(guān)鍵詞：煙草；茄尼醇；主基因+多基因；遺傳效應(yīng)
　　中圖分類號：S572.03 文章編號：1007-5119（2015）06-0001-07DOI：10.13496/j.issn.1007-5119.2015.06.001
　　Genetic Analysis on Solanesol Content ofTobacco
　　XIANG Dehu1，2， ZHAO Taozhi3， DU Yongmei1， ZHANG Zhongfeng1， YAN Ning1， HUANG Wenchang4，
　　WANG Aihua1，F(xiàn)U Qiujuan1， GONG Yanan1， LIU Yanhua1*
　�。�1. Key Laboratory of Tobacco Genetic Improvement and Biotechnology， Tobacco Research Institute of Chinese Academy of Agricultural Sciences， Qingdao 266101， China； 2. Graduate School of Chinese Academy of Agricultural Sciences， Beijing 100081， China； 3. Northeast Agricultural University College of Life Science， Harbin 150030， China； 4. Tobacco Research Institute of Hubei Province， Wuhan 430030， China）
　　Abstract：To explore thegenetic model of solanesol content of tobacco leaf，a cross was made with the low solanesol Maryland609 as maternal parent and the high solanesol K326 as paternal parent andpopulations of four generations （P1， P2， F1， F2） were obtained. Determination of solanesol content of the four populations was donewith UPLC. Genetic analysis of solanesol content in tobacco was carried out using the joint segregation analysis method with amixed genetic model of major gene plus polygene.The results showed that solanesol content of tobacco leaf appeared to be a quantitative trait and the inheritance fit to a mixed genetic model of two majorgenes with equal additive effects plus polygenes with additive-dominant effects （E6 model）.The results from two years showed that the additive effects and dominant effects of two major genes were equal and the quantitative value was negative.Dominant effects of polygenes were greater than additive effects. Heritabilities of the major genes were estimated to be 33.61% and 53.15%， respectively. Heritabilities of the polygenes were estimated to be 30.01% and 13.64%， respectively.The value of environmental variation was between 33.21%-36.28%. The results indicated that solanesol content of tobacco leaf was mainly controlled by major genes and poly genes， in which the major genes were dominant， and was effected by environmental factors at the same time.

相關(guān)熱詞搜索：遺傳 煙草 含量 分析 茄尼醇