蒙藥手參藥材與奶手參的質(zhì)量標(biāo)準(zhǔn)

　　摘要 目的：對蒙藥手參藥材和奶手參（奶制飲片）進(jìn)行系統(tǒng)性生藥學(xué)鑒定及分析，為其質(zhì)量標(biāo)準(zhǔn)的建立及鑒定提供科學(xué)依據(jù)。方法：參照《中華人民共和國藥典》（2010年版附錄）相關(guān)方法，對手參藥材和奶手參的水分，灰分及浸出物進(jìn)行測定；采用顯微，薄層色譜法進(jìn)行定性鑒別。以天麻素為對照品，用70%甲醇提取，以乙酸乙酯-甲酸-水（9∶1∶0.2）為展開劑，噴以10%磷鉬酸乙醇溶液，在105 ℃加熱至斑點顯色清晰。采用紫外-可見分光光度法和高效液相色譜法對手參與奶手參中的多糖及天麻素進(jìn)行含量分析。色譜柱為ZOBB AX eclipse XDB-C18（4.6 mm×250 mm，5 cm）柱；以甲醇-0.04%的磷酸溶液（8∶92）為流動相，流速為1.0 mL/min；檢測波長222 nm；柱溫30 ℃。結(jié)果：薄層色譜法試驗中在與對照品色譜相應(yīng)的位置上，顯相同顏色的斑點。高效液相色譜條件下天麻素被洗脫且達(dá)到基線分離，天麻素在濃度0.009～0.09 mg/mL范圍內(nèi)線性關(guān)系良好，回歸方程為Y=586866X+425821（R=0.9996），平均回收率為100.1%。精密度試驗、重復(fù)性試驗、穩(wěn)定性試驗均符合要求。三批樣品浸出物結(jié)果為26.13%～42.58%，水分為3.47%～5.31%，總灰分為5.43%～6.33%。結(jié)論：質(zhì)控方法可靠，靈敏度高，專屬性，準(zhǔn)確，穩(wěn)定性好。該結(jié)果對手參藥材鑒定、資源利用及質(zhì)量標(biāo)準(zhǔn)提升，提供科學(xué)依據(jù)。
　　關(guān)鍵詞 蒙藥；手參；顯微鑒別；薄層色譜法；高效液相色譜法；質(zhì)量標(biāo)準(zhǔn)
　　Abstract Objective：To carry out a systematic pharmacognostical study on Shoushen and Naishoushen， and to provide the basis for establishing its quality standard and appraisal. Methods：Based on the methods recorded in appendix of Pharmacopoeia of the People′s Republic of China （2010 edition， volume1）， the water content， total ash and ethanol-soluble extractives of the crude drugs were detected. The microscopy TLC was used for qualitative identification. With gastrodin as control agent， 70% methyl alcohol was used to extract. With ethyl acetate formic acid-water （9∶1∶0.2） as developing solvent， 10% phosphomolybdic acid ethanol solution was sprayed. And at 105 ℃ heated until spot color was clear. By ultraviolet-visible spectrophotometry and HPLC method were used for the determination of contents of the polysaccharides and gastrodine of Shoushen and Naishoushen. Chromatographic column for ZOBB AX eclipse XDB-C18 （4.6 mm×250 mm， 5 cm）； methanol-water （0.04% phosphoric acid） （8∶92） was used as the mobile phase at a flow rate of 1.0 mL/min. The column temperature was 30 ℃ and the detection wavelength was at 222 nm. Results：In thin layer chromatography （TLC）， the same color spots were detected in the corresponding positions of the chromatography. HPLC was used to elute the gastrodine and achieve the baseline separation. The concentration of gastrodine in the concentration range 0.009～0.09 mg/mL was good. And the regression equation was Y=586866X+425821 （R=0.9996） and the average recovery was 100.1%. Precision test， repeatability test， stability test were in line with the requirements. Three batches of sample extract was 26.13%～42.58%， water 3.47%～5.31% and total ash content was 5.43%～6.33%. Conclusion：The indictment method is reliable， sensitive， specific， accurate and stable. The results provide scientific basis for identification， resource utilization and improvement of quality standards.

相關(guān)熱詞搜索：蒙藥 藥材 質(zhì)量標(biāo)準(zhǔn) 奶手參