泛耐藥鮑氏不動(dòng)桿菌氨基糖苷類耐藥相關(guān)基因研究

　　摘   要：目的  探討泛耐藥鮑氏不動(dòng)桿菌氨基糖苷類抗菌藥物耐藥機(jī)制。方法  收集2014年湖北省黃石市中心醫(yī)院住院患者中分離的20株泛耐藥鮑氏不動(dòng)桿菌，用mdfA測(cè)序確認(rèn)菌種，再采用聚合酶鏈反應(yīng)法分析16種氨基糖苷類藥物獲得性耐藥相關(guān)基因。 結(jié)果  20株泛耐藥鮑氏不動(dòng)桿菌共檢出4種氨基糖苷類獲得性耐藥基因，氨基糖苷類修飾酶基因aac（3）-Ⅰ陽(yáng)性率15.00%;ant（3"）-Ⅰ陽(yáng)性率20.00%;aph3"-Ⅰ陽(yáng)性率100.00%;16SrRNA甲基化酶未檢出;外排泵adeB基因陽(yáng)性率95.00%。 結(jié)論 泛耐藥鮑氏不動(dòng)桿菌中攜帶的耐藥基因和耐藥表型相對(duì)應(yīng)，對(duì)氨基糖苷類藥物耐藥與產(chǎn)aac（3）-Ⅰ、ant（3"）-Ⅰ、aph3"-Ⅰ修飾酶基因和獲得外排泵adeB 2種耐藥機(jī)制有關(guān)。
　　關(guān)鍵詞：鮑氏不動(dòng)桿菌;氨基糖苷類修飾酶基因;外排泵基因;泛耐藥
　　中圖分類號(hào)：R378                                  文獻(xiàn)標(biāo)識(shí)碼：A                                  DOI：10.3969/j.issn.1006-1959.2018.05.025
　　文章編號(hào)：1006-1959（2018）05-0078-03
　　Study on Aminoglycoside Pan Resistance Related Genes of Acinetobacter Baumannii
　　BAO Qun-li，KE Jun，HU Fang，ZHANG Li-Hong，HUANG Yan，CHENG Pan，PEN Zi-Tan，WANG Hong-Liang
　�。―epartment of Clinical Laboratory，Huangshi Central Hospital，Huangshi 435000，Hubei，China）
　　Abstract：Objective  To investigate the mechanism of aminoglycoside Pan resistance of Acinetobacter baumannii.Methods  20 strains of Pan resistance Acinetobacter baumannii isolated from hospitalized patients in Huangshi central hospital of Hubei province in 2014 were identified by mdfA sequencing.Polymerase chain reaction was used to analyze the acquired resistance related genes of 16 aminoglycosides.Results  A total of 4 aminoglycoside-resistant Acinetobacter baumannii strains were detected in 20 Pan-resistant Acinetobacter baumannii strains.The aminoglycoside modifying enzyme gene aac（3）-I was positive in 15.00%;ant（3"）-I The positive rate was 20.00%;aph3"-I，the positive rate was 100.00%;16S rRNA methylase was not detected;the positive rate of adeB gene of the efflux pump was 95.00%.Conclusion  The drug-resistance genes in pan-resistant Acinetobacter baumannii correspond to drug resistance phenotypes，and they are resistant to aminoglycosides and produce aac（3）-I，ant（3"）-I，aph3"-I the modification of the enzyme gene and the acquisition of efflux pump adeB are related to two drug resistance mechanisms.
　　Key words：Acinetobacter baumannii;Aminoglycoside modifying enzyme gene;Efflux pump gene;Pan-resistance
　　繼臨床分離到多藥耐藥的鮑氏不動(dòng)桿菌（MDRAB）后，更嚴(yán)重的泛耐藥鮑氏不動(dòng)桿菌（XDRAB）與極度耐藥鮑氏不動(dòng)桿菌（PDRAB）在國(guó)內(nèi)外都有分離報(bào)告[1-3]。為全面了解XDRAB氨基糖苷類抗菌藥物耐藥機(jī)制，我們對(duì)分離自湖北省黃石市中心醫(yī)院20株XDRAB進(jìn)行了9種氨基糖苷類修飾酶基因、6種16SrRNA甲基化酶基因以及外排泵adeB基因檢測(cè)，現(xiàn)報(bào)道如下。
　　1資料與方法

相關(guān)熱詞搜索：耐藥 糖苷 氨基 不動(dòng) 桿菌