經(jīng)關(guān)節(jié)穿刺注射ADSCs修復(fù)兔激素性股骨頭壞死的研究

　　[摘要] 目的 探討經(jīng)關(guān)節(jié)穿刺注射脂肪源性干細(xì)胞（ADSCs）修復(fù)兔激素性股骨頭缺血性壞死的療效。 方法 取成年新西蘭大白兔頸背部脂肪組織提取脂肪源性干細(xì)胞培養(yǎng)并傳代，36只新西蘭大白兔通過馬血清聯(lián)合激素法建立激素性股骨頭缺血性壞死模型，隨機分為對照組、髓芯減壓組及ADSCs組，每組各12只，并作骨密度檢測。對照組不作干預(yù)處理，髓芯減壓組股骨頭鉆孔減壓，ADSCs組經(jīng)關(guān)節(jié)穿刺注射ADSCs。分別于干預(yù)后4、8周每組取6只兔行骨密度檢測及X線檢查，處死后收集股骨頭樣本作大體觀察，采用HE染色及PCR檢測BMP-2 mRNA的表達，通過各組比較探討經(jīng)關(guān)節(jié)穿刺注射ADSCs修復(fù)激素性股骨頭缺血性壞死的效果。 結(jié)果 干預(yù)后4、8周，三組骨密度呈下降趨勢，但對照組下降幅度更大，與髓芯減壓組、ADSCs組比較差異有統(tǒng)計學(xué)意義（P < 0.05），而髓芯減壓組與ADSCs組相比較差異無統(tǒng)計學(xué)意義（P > 0.05）；大體觀察、影像學(xué)及組織學(xué)表現(xiàn)，干預(yù)后4、8周與對照組比較，經(jīng)關(guān)節(jié)穿刺注射ADSCs及髓芯減壓均可延緩股骨頭壞死的進展，對股骨頭壞死起修復(fù)作用，ADSCs組優(yōu)于髓芯減壓組；干預(yù)后4、8周，ADSCs組及髓芯減壓組BMP-2 mRNA的表達水平均高于對照組，差異有統(tǒng)計學(xué)意義（P < 0.05），而髓芯減壓組與ADSCs比較差異無統(tǒng)計學(xué)意義（P > 0.05）。 結(jié)論 經(jīng)關(guān)節(jié)穿刺注射ADSCs對兔激素性股骨頭缺血性壞死有修復(fù)作用。
　　[關(guān)鍵詞] 激素性股骨頭缺血性壞死；脂肪源性干細(xì)胞；關(guān)節(jié)穿刺；修復(fù)
　　[中圖分類號] R6874 [文獻標(biāo)識碼] A [文章編號] 1673-7210（2018）03（a）-0175-06
　　Repair of rabbit steroid-induced avascular necrosis of femoral head by ADSCs injection through the joint puncture
　　TAN Weiyuan AN Rongze QI Xinwen XIE Jiaqing
　　Department of Orthopedics， the Fifth Affiliated Hospital of Zunyi Medical College， Guangdong Province， Zhuhai 519100， China
　　[Abstract] Objective To investigate the effect of adipose derived stem cells （ADSCs） injection through the joint puncture on the repair of steroid-induced avascular necrosis of femoral head in rabbits. Methods ADSCs were isolated and passaged from the neck adipose tissue of adult New Zealand white rabbits. Thirty-six New Zealand white rabbits were used to establish the model of steroid-induced avascular necrosis of femoral head by horse serum combined with hormone. These rabbits were randomly divided into control group， core decompression group and ADSCs group （12 rabbits in each group）， and all for bone density detection. Control group：no intervention； core decompression group： femoral head drilling decompression； ADSCs group：by intra-articular injection of ADSCs. At 4 weeks and 8 weeks after intervention， 6 rabbits in each group were examined with bone mineral density （BMD） and X-ray examination. After the death， their femur samples were collected for gross observation， HE staining and PCR were used for the measurement of BMP-2 mRNA. Through the comparison of each group to explore the effect of ADSCs repairing steroid-induced avascular necrosis of the femoral head by joint puncture injection. Results 4 weeks and 8 weeks after intervention three groups of BMD， but the control group decreased， compared with core decompression group and ADSCs group were statistically significant （P < 0.05）， but there was no significant difference between the core decompression group and the ADSCs group （P > 0.05）. General observation， imaging and histological findings at 4 weeks and 8 weeks after intervention， compared with the control group， ADSCs treatment by joint puncture injection and core decompression both can delay the progress of femoral head necrosis. There had a repair effect on femoral head necrosis， and the effects in ADSCs group were better than those in core decompression group； at 4 weeks and 8 weeks after intervention， the expression of BMP-2 mRNA in the ADSCs group and the core decompression group were both higher than those in the control group， the differences were statistically significant （P < 0.05）， while there was no significant difference between the core decompression group and the ADSCs group （P > 0.05）. Conclusion ADSCs injected through the joint puncture can repair the steroid induced avascular necrosis of the femoral head in rabbits.

相關(guān)熱詞搜索：穿刺 激素 注射 關(guān)節(jié) 股骨頭壞死