高烏頭藥材常規(guī)項(xiàng)檢查及高烏甲素、冉烏頭堿含量測(cè)定

　　摘要：目的 對(duì)高烏頭藥材進(jìn)行常規(guī)項(xiàng)檢查并測(cè)定高烏甲素、冉烏頭堿含量，為建立高烏頭藥材質(zhì)量標(biāo)準(zhǔn)提供參考。方法 采集不同產(chǎn)地高烏頭藥材，采用薄層色譜法進(jìn)行定性鑒別，按藥典水分測(cè)定法（烘干法）、灰分測(cè)定法、浸出物測(cè)定法測(cè)定水分、灰分和浸出物，采用HPLC測(cè)定高烏甲素和冉烏頭堿含量。結(jié)果 薄層色譜斑點(diǎn)清晰，分離度好；暫定高烏頭藥材中水分、總灰分及酸不溶性灰分分別不得過(guò)11.0%、12.0%、7.0%，水溶性浸出物和醇溶性浸出物分別不少于18.2%、10.6%；高烏頭藥材中冉烏頭堿和高烏甲素含量分別不少于0.125%、0.815%。結(jié)論 本研究所建立的方法準(zhǔn)確可靠，可用于高烏頭藥材質(zhì)量評(píng)價(jià)。
　　關(guān)鍵詞：高烏頭；常規(guī)檢查；高效液相色譜法；高烏甲素；冉烏頭堿
　　DOI：10.3969/j.issn.1005-5304.2017.11.015
　　中圖分類號(hào)：R284.1 文獻(xiàn)標(biāo)識(shí)碼：A 文章編號(hào)：1005-5304（2017）11-0063-04
　　Routine Examination of Aconitum sinomontanum Nakai and Content Determination of Lappacontine and Ranaconitine ZHANG Li-jun1， DAI Hai-rong1， FAN Qin2， XIA Peng-fei2， SHEN Bing-xue1， LI Yun1 （1. Gansu University of Chinese Medicine， Lanzhou 730000， China； 2. Key Laboratory of Chemistry and Quality for Traditional Chinese （Tibetan） Medicines of Colleges of Gansu Province， Lanzhou 730000， China）
　　Abstract： Objective To study the routine examination of Aconitum sinomontanum Nakai and determine the contents of lappacontine and ranaconitine； To provide basis for establishing the quality standard of Aconitum sinomontanum Nakai. Methods Aconitum sinomontanum Nakai were collected from different areas. A method of TLC was used for qualitative discrimination. The methods in the Chinese Pharmacopoeia were adopted for the determination of moisture content， ash content and extractives. Determination of lappacontine and ranaconitine were performed by HPLC. Results The TLC showed that the spots were clear and the separation was good. Individual provisional standards： the moisture， total ash and acid-insoluble ash content of Aconitum sinomontanum Nakai were not more than 11.0%， 12.0%， and 7.0%， respectively； water soluble and alcohol soluble extractives were not less than 18.2% and 10.6%， respectively. The content of ranaconitine and lappacontine in Aconitum sinomontanum Nakai were not less than 0.125% and 0.815%， respectively. Conclusion The method established by the study is accurate and reliable， and can be used for quality evaluation of Aconitum sinomontanum Nakai.
　　Key words： Aconitum sinomontanum Nakai； routine examination； HPLC； lappacontine； ranaconitine
　　高烏頭為毛茛科烏頭屬植物高烏頭Aconitum sinomontanum Nakai的根，又名麻布七、辮子七、破骨七等，是我國(guó)甘肅等中西部地區(qū)民間習(xí)用中藥，也是著名“七藥”之一[1]，具有祛風(fēng)除濕、理氣止痛、活血散瘀的功效，用于治療風(fēng)濕痹痛、關(guān)節(jié)腫痛、跌打勞傷等�，F(xiàn)代藥理研究表明，高烏頭具有鎮(zhèn)痛、抗炎、解熱、局部麻醉、抗心律失常、抗氧化、殺蟲等廣泛的藥理作用[2-4]，臨床上常用于治療類風(fēng)濕關(guān)節(jié)
　　基金項(xiàng)目：國(guó)家自然科學(xué)基金（81560650）；甘肅省自然科學(xué)基金（1107RJZA242）
　　通訊作者：李蕓，E-mail：liyunherb@163.com
　　炎及局部鎮(zhèn)痛[5-6]。高烏頭的主要活性成分為二萜生物堿[7]，其中以高烏甲素和冉烏頭堿的含量最高[8]，而活性成分之一高烏甲素已開發(fā)出不同劑型并應(yīng)用于臨床[9]。

相關(guān)熱詞搜索：烏頭 測(cè)定 藥材 含量 常規(guī)