香煙煙霧提取物對(duì)小鼠C2C12細(xì)胞myostatin及炎癥介質(zhì)的影響

　　[摘要]目的 探討香煙煙霧提取物（CSE）對(duì)小鼠C2C12細(xì)胞肌肉生長(zhǎng)抑制素（myostatin）及炎癥介質(zhì)的影響。方法 不同濃度CSE和（或）myostatin-siRNA刺激和（或）干擾小鼠C2C12細(xì)胞后利用MTT檢測(cè)CSE對(duì)細(xì)胞生長(zhǎng)的影響，根據(jù)處理方式不同，實(shí)驗(yàn)分為4個(gè)組，分別為對(duì)照組、5% CSE組、myostatin-siRNA組、5% CSE+myostatin-siRNA組，采用Real-time PCR檢測(cè)myostatin mRNA表達(dá)情況，采用Western blot檢測(cè)myostatin蛋白表達(dá)情況，采用ELISA檢測(cè)細(xì)胞培養(yǎng)液中腫瘤壞死因子-α（TNF-α）和白細(xì)胞介素8（IL-8）含量。結(jié)果 5%以上的CSE處理能顯著抑制小鼠C2C12細(xì)胞增殖（P<0.05）；5%的CSE處理小鼠C2C12細(xì)胞48 h后，myostatin mRNA和蛋白表達(dá)明顯高于對(duì)照組（P<0.05），細(xì)胞培養(yǎng)液中IL-8和TNF-α含量明顯高于對(duì)照組（P<0.05）；siRNA干擾myostatin后再采用CSE處理小鼠C2C12細(xì)胞，細(xì)胞培養(yǎng)液中IL-8和TNF-α含量明顯低于CSE處理組（P<0.05）。結(jié)論 CSE上調(diào)小鼠C2C12細(xì)胞myostatin表達(dá)，增加炎癥介質(zhì)釋放。
　　[關(guān)鍵詞]慢性阻塞性肺疾��；香煙煙霧提取物；肌肉生長(zhǎng)抑制素；炎癥介質(zhì)
　　[中圖分類號(hào)] R332 [文獻(xiàn)標(biāo)識(shí)碼] A [文章編號(hào)] 1674-4721（2018）2（b）-0004-04
　　Influence of cigarette smoke extract on myostatin and inflammatory mediators in mouse C2C12 cells
　　LI Yu-ji ZHOU Xu PEI Xing-hua HUANG Li LEI Yan-jun ZHANG Lu-lu
　　The First Department of Critical Care Medicine，Peopel′s Hospital of Hu′nan Province，Changsha 410005，China
　　[Abstract]Objective To investigate the effect of cigarette smoke extract on myostatin and inflammatory mediators in mouse C2C12 cells.Methods Different concentrations of CSE and/or myostatin-siRNA stimulated or interference mouse C2C12 cells，and then detected influence of CSE on cell growth by MTT，according to the different treatment methods，the experimental research objects were divided into the control group，5% CSE group，the myostatin-siRNA group，5% CSE+myostatin-siRNA group.Real-time PCR was used to detect the expression of myostatin mRNA，Western blot was used to detect expression of myostatin protein，ELISA was used to detected tumor necrosis factor alpha （TNF-alpha） and interleukin 8 （IL-8） level in cell culture medium.Results The CSE at the concentration above 5% could significantly inhibit the proliferation of C2C12 cells in mice （P<0.05）.The expression of myostatin mRNA and protein in C2C12 cells treated with 5% CSE was significantly higher than that of the control group after 48 h （P<0.05）.The content of IL-8 and TNF-alpha in the cell culture fluid was significantly higher than that in the control group （P<0.05）.After siRNA interfered with myostatin，the mouse C2C12 cells were treated with CSE，the content of IL-8 and TNF-alpha in cell culture solution was significantly lower than that in CSE treatment group （P<0.05）.Conclusion CSE can up-regulate the expression of myostatin and increase inflammatory mediators releasing from C2C12 cells.
　　[Key words]Chronic obstructive pulmonary disease；Cigarette smoke extract；Myostatin；Inflammatory mediator

相關(guān)熱詞搜索：小鼠 提取物 炎癥 介質(zhì) 煙霧